Biomphalaria tenagophila potencial vector of Schistosoma mansoni in the Paraná River basin (Argentina and Paraguay).

Susceptibility and compatibility experiments were carried out with 700 Biomphalaria tenagophila from the Paraná River basin exposed to infection with Schistosoma mansoni. Individual infection was performed with 10 miracidia of SJ2 strain from the Paraiba valley (Brazil) originally infective to B. tenagophila. These snails were laboratory-breed progeny of B. tenagophila collected from six localities of Argentina and one from Paraguay. From Argentina: Rincón de Vences (7%) and Posadas (11%) became infected with S. mansoni and the calculation of Frandsen's index (TCP/100) shows that they were Class II poorly compatible. Those snails from Goya (22%), Maloyas (5%), and Berón de Astrada (3%) were Class III compatible to the S. mansoni. None of the 100 snails exposed from Caá-Catí became infected (Class 0 incompatible). Tested samples from Paraguay (Encarnación) were infected (20%) and compatible (Class III). It was also studied the persistence of the infection in 244 snails of the first generation (F1) of those that were susceptible from three places. It was demonstrated an increment of the susceptibility in the F1 from Maloyas (chi2 = 27.22; p = 0.0001) and Posadas (chi2 = 4.24; p = 0.04). The results point out the possibility that schistosomiasis might be able to spread into the Paraná River basin where B. tenagophila exists.

Biomphalaria tenagophila potencial vector of Schistosoma mansoni in the Paraná River basin (Argentina and Paraguay)

Susceptibility and compatibility experiments were carried out with 700 Biomphalaria tenagophila from the Paraná River basin exposed to infection with Schistosoma mansoni. Individual infection was performed with 10 miracidia of SJ2 strain from the Paraiba valley (Brazil) originally infective to B. tenagophila. These snails were laboratory-breed progeny of B. tenagophila collected from six localities of Argentina and one from Paraguay. From Argentina: Rincón de Vences (7 percent) and Posadas (11 percent) became infected with S. mansoni and the calculation of Frandsen's index (TCP/100) shows that they were Class II poorly compatible. Those snails from Goya (22 percent), Maloyas (5 percent), and Berón de Astrada (3 percent) were Class III compatible to the S. mansoni. None of the 100 snails exposed from Caá-Catí became infected (Class 0 incompatible). Tested samples from Paraguay (Encarnación) were infected (20 percent) and compatible (Class III). It was also studied the persistence of the infection in 244 snails of the first generation (F1) of those that were susceptible from three places. It was demonstrated an increment of the susceptibility in the F1 from Maloyas (chi2 = 27.22; p = 0.0001) and Posadas (chi2 = 4.24; p = 0.04). The results point out the possibility that schistosomiasis might be able to spread into the Paraná River basin where B. tenagophila exists.

Intermediate and definitive hosts of Schistosoma mansoni in Corrientes province, Argentina

Corrientes province is located in the humid subtropical region of Argentina northeast on the left riverbank of Paraná River in the border with the South of Brazil. This is a region without schistosomiasis but planorbid and rodents that would serve as host of the life cycle of Schistosoma mansoni inhabit here. The objective of this work is to know the role of rodent as definitive host of schistosomiasis. Biomphalaria tenagophila (4 to 8 mm Ø) from Maloyas, exposed each to 10 miracidia of SJ2 strain of S. mansoni natives from Brazil were susceptible (5 percent). The degree of compatibility was Class II of Frandsen. Five wild rodents captured in the same ecological niche were exposed transcutaneously to infection with 40 cercariae for animal: two Olygoryzomys flavescens, two Holochilus braziliensis, and one Scapteromys tuncidus. Only one H. braziliensis eliminated eggs in feces. Prepatent period was of 83 days. With these feces, two of six (33.3 percent) B. tenagophila from Maloyas were infected with miracidium. It was demonstrated, in an area free of schistosomiasis, that life cycle S. mansoni is closed with planorbid and rodents that live in the same ecological niche.

Intermediate and definitive hosts of Schistosoma mansoni in Corrientes province, Argentina.

Corrientes province is located in the humid subtropical region of Argentina northeast on the left riverbank of Paraná River in the border with the South of Brazil. This is a region without schistosomiasis but planorbid and rodents that would serve as host of the life cycle of Schistosoma mansoni inhabit here. The objective of this work is to know the role of rodent as definitive host of schistosomiasis. Biomphalaria tenagophila (4 to 8 mm Ø) from Maloyas, exposed each to 10 miracidia of SJ2 strain of S. mansoni natives from Brazil were susceptible (5%). The degree of compatibility was Class II of Frandsen. Five wild rodents captured in the same ecological niche were exposed transcutaneously to infection with 40 cercariae for animal: two Olygoryzomys flavescens, two Holochilus braziliensis, and one Scapteromys tuncidus. Only one H. braziliensis eliminated eggs in feces. Prepatent period was of 83 days. With these feces, two of six (33.3%) B. tenagophila from Maloyas were infected with miracidium. It was demonstrated, in an area free of schistosomiasis, that life cycle S. mansoni is closed with planorbid and rodents that live in the same ecological niche.

Species assignation of Leishmania from human and canine American tegumentary leishmaniasis cases by multilocus enzyme electrophoresis in North Argentina.

Sixteen Leishmania stocks, 15 isolated from patients with cutaneous (CL), mucocutaneous (MCL), or recurrent cutaneous leishmaniasis, plus one from a dog with CL in Salta and Corrientes Provinces, Argentina, were studied by multilocus enzyme electrophoresis. Thirteen of the stocks from humans were grouped in two zymodemes; nine termed as KMS 1, four as KMS 2, and assigned to Leishmania (Viannia) braziliensis. Two additional stocks from CL cases expressed a KMS 4 enzyme profile, corresponding to L. (V.) guyanensis. Although the parasites from the dog were also assigned to L. (V.) braziliensis, its zymodeme, KMS 3, was not expressed in any of the current human isolates. The characterization of Leishmania from a dog was done for the first time in Argentina. The importance of the intraspecific polymorphism in the induction of clinical forms and in the host-reservoir concept is briefly discussed, based on the zymodeme data of isolates from humans and dogs. The presence of L. (V.) guyanensis was confirmed in the country.

Parasitismo intestinal en San Cayetano, Corrientes, Argentina / Intestinal parasitism in San Cayetano, Corrientes, Argentina

En San Cayetano, aldea de la provincia de Corrientes, Argentina, se llevó a cabo una investigación epidemiológica para determinar la prevalencia de parasitosis intestinales en niños. Se eligieron aleatoriamente 88 viviendas y se sometieron a examen microscópico las muestras fecales, recolectadas durante 6 días consecutivos, de 207 niños (72 porciento de la población en edad escolar y 12 porciento de la población total de la aldea). De las muestras examinadas, 170 (83 porciento) tuvieron uno o más parásitos, de los cuales el más frecuente fue Blastocystis hominis (43 porciento). También se hallaron Giardia lamblia (29 porciento), uncinarias (27 porciento), Entamoeba coli (27 porciento), Enterobius vermicularis (3 porciento), Strongyloides stercoralis (2 porciento), Ascaris lumbrocoides, Trichuris trichiura, Taenia saginata, Isospora belli, Balantidium coli y Iodamoeba butschlii (0,5 porciento). La alta prevalencia de parasitosis intestinales indica que en San Cayetano hay una transmisión activa de parásitos como consecuencia de la mala higiene ambiental, particularmente la falta de agua potable, de una red de alcantarillado y de un sistema de recolección domiciliaria de basuras

Parasitismo intestinal en San Cayetano, Corrientes, Argentina / Intestinal parasitism in San Cayetano, Corrientes, Argentina

En San Cayetano, aldea de la provincia de Corrientes, Argentina, se llevó a cabo una investigación epidemiológica para determinar la prevalencia de parasitosis intestinales en niños. Se eligieron aleatoriamente 88 viviendas y se sometieron a examen microscópico las muestras fecales, recolectadas durante 6 días consecutivos, de 207 niños (72 porciento de la población en edad escolar y 12 porciento de la población total de la aldea). De las muestras examinadas, 170 (83 porciento) tuvieron uno o más parásitos, de los cuales el más frecuente fue Blastocystis hominis (43 porciento). También se hallaron Giardia lamblia (29 porciento), uncinarias (27 porciento), Entamoeba coli (27 porciento), Enterobius vermicularis (3 porciento), Strongyloides stercoralis (2 porciento), Ascaris lumbrocoides, Trichuris trichiura, Taenia saginata, Isospora belli, Balantidium coli y Iodamoeba butschlii (0,5 porciento). La alta prevalencia de parasitosis intestinales indica que en San Cayetano hay una transmisión activa de parásitos como consecuencia de la mala higiene ambiental, particularmente la falta de agua potable, de una red de alcantarillado y de un sistema de recolección domiciliaria de basuras

Se publica en inglés en el Bulletin PAHO Vol. 30(2), 1996